Application DetailsELISA. Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Scientific BackgroundPeptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Analysis of PYY in Sandwich ELISA. Coating antibody was Chicken polyclonal antibody to human Peptide YY (C-1536-100) at 1:400. Detection antibody was Chicken polyclonal antibody to PYY biotinylated (C-1539-100) at 1:5,000.