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Choline O-acetyltransferase (ChAT), Rabbit Polyclonal Antibody
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Catalog Number
R-043
- Product Name Choline O-acetyltransferase (ChAT), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Choline O-acetyltransferase (ChAT) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC, ELISA.
- Alternative Names Choline O-acetyltransferase; CHOACTase; Choline acetylase
- Application(s) ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit.
- Species Reactivity Guinea Pig, Mouse, Pig, Rabbit, Rat
- Immunogen Description A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
- Conjugate Unconjugated
- Purity Description Whole serum
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Choline O-acetyltransferase (ChAT) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC, ELISA.
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Related Products
Choline O-acetyltransferase (ChAT) control peptide (167-188), Purified Peptide
- Application(s) ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded
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Application Details
Immunohistochemistry/cytochemistry on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times
ELISA: direct antigen ELISA
This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. - Target Choline O-acetyltransferase (ChAT)
- Specificity This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit.
- Target Host Species Pig
- Species Reactivity Guinea Pig, Mouse, Pig, Rabbit, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype Mixed
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
- Purity Description Whole serum
- Format Lyophilized
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Choline O-acetyltransferase; CHOACTase; Choline acetylase
- Uniprot Number P13222
- Uniprot Number/Name P13222 (CLAT_PIG)
- Scientific Background This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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Specific References
Beig MI, Dampney BW and Carrive P (2014) Both ox1r and ox2r orexin receptors contribute to the cardiovascular and locomotor components of the novelty stress response in the rat. Neuropharmacology September 16[Epub ahead of print] Application: IH; Species: Rat
Ellis KM, O'Carroll DC, Lewis MD, Rychkov GY, Koblar SA (2014) Neurogenic potential of dental pulp stem cells isolated from murine incisors. Stem Cell Res Ther. 2014 Feb 27;5(1):30.Application: IF; Species: Mouse
Leong WK, Klaric TS, Lin Y, Lewis MD, Koblar SA (2013) Upregulation of the neuronal Per-Arnt-Sim domain protein 4 (Npas4) in the rat corticolimbic system following focal cerebral ischemia. Eur J Neurosci. 2013 Jun;37(11):1875-84Application: IH; Species: Rat
Li S et al (2011) The expression and localization of Prune2 mRNA in the central nervous system. Neurosci Lett. Oct 10;503(3):208-14.
- General References Benecke et al. (1993). J. Neurochemistry. 61: 804-811