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Dopamine beta-hydroxylase (DBH), Rabbit Polyclonal Antibody
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Catalog Number
R-108
- Product Name Dopamine beta-hydroxylase (DBH), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Dopamine beta-hydroxylase (DBH) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, Neutralize.
- Alternative Names Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase;
- Application(s) IHC-Frozen, Neutralize, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.
- Species Reactivity Bovine, Guinea Pig, Human, Rat
- Immunogen Description Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.
- Conjugate Unconjugated
- Purity Description Whole serum
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Dopamine beta-hydroxylase (DBH) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, Neutralize.
- Application(s) IHC-Frozen, Neutralize, WB
- Application Details IHC (frozen), Western Blot, radioimmunoassay and inhibition of enzymatic activity. Recommended to be used at a dilution of 1:500 to 1: 2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Dopamine beta-hydroxylase (DBH)
- Specificity This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.
- Target Host Species Bovine
- Species Reactivity Bovine, Guinea Pig, Human, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype Mixed
- Conjugate Unconjugated
- Immunogen Description Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.
- Purity Description Whole serum
- Format Lyophilized
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase;
- Uniprot Number P15101
- Uniprot Number/Name P15101 (DOPO_BOVIN)
- Scientific Background DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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Specific References
Bowolaksono A. (2012) Molecular Regulation of Noradrenaline in Bovine Corpus Luteum. HAYATI Journal of Biosciences June 2012 Vol. 19 No. 2, p 81-87
Blanco-Centurion C. et al (2004) Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone. Eur J Neurosci. 2004 May;19(10):2741-52.
Ohshita N. et al (2004) Characteristics of the trigeminal depressor response in cats. J Neurosci Res. 2004 Jun 15;76(6):891-901.
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General References
Rush R. A. et al (1980) Gut Rev clin Lab Sci 12: 241
Verndell I. M. et al (1982) J Histochem Cytochem 30: 682