Product NamePhospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s)WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityRat Predicted from gene analysis to react with human and mouse alpha-CaMKII.
Species ReactivityRat
Immunogen DescriptionA synthetic peptide (NKmLpTINPSC) corresponding to the sequence around Thr253 (AA 249-257) in alpha-CaMKII was synthesized, purified to 95% purity by HPLC, analyzed by mass spectroscopy and coupled to diphtheria toxoid.
Product DescriptionRabbit anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s)WB
Application DetailsWestern Blotting (1:200 - 1:1000). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetPhospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253)
SpecificityRat Predicted from gene analysis to react with human and mouse alpha-CaMKII.
Target Host SpeciesRat
Species ReactivityRat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (NKmLpTINPSC) corresponding to the sequence around Thr253 (AA 249-257) in alpha-CaMKII was synthesized, purified to 95% purity by HPLC, analyzed by mass spectroscopy and coupled to diphtheria toxoid.
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCalcium/calmodulin-stimulated protein kinase II (CaMKII) is composed of four different chains (alpha, beta, gamma, and delta) and is abundantly expressed in neurons. CaMKII is involved in regulating many aspects of neuronal function, including neurotransmitter synthesis and release, modulation of ion channel activity and cellular transport. The enzymatic function of CaMKII is regulated by its multiple phosphorylation sites and targeting to sub-cellular locations through interactions with protein binding partners. Phosphorylation of Thr253 has been identified in vivo and found to alter the interaction of CaMKII with binding partners, but not change its enzymatic activity. Thus, phosphorylation of Thr253 is suggested to modulate functional responses based on its binding partner and subsequently its sub-cellular localization.
Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.
Western blots of wild-type CaMKII showing auto-phosphorylation over 4 min at 10 µM ATP, 30°C and probed with antibodies to α-CaMKII, phospho-Thr286-CaMKII (anti-pT286) and phospho-Thr253-CaMKII (anti-pT253). The arrow-heads indicate the electrophoretic mobility of CaMKII phosphorylated at Thr253. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.
Specific ReferencesSkelding KA et al (2012) J J Cereb Blood Flow Metab 32 (12), 2181-2192. Skelding KA et al (2010) Cell Signal 22 (5), 759-769. Gurd JW et al (2008) Brain Res 1218, 158-165. Migues PV et al (2006) J Neurochem 98 (1), 289-299.
General ReferencesSkelding KA et (2011) Cell Cycle 10 (4), 631-63 Dosemeci A & Jaffe H (2010) Biochem Biophys Res Commun 391 (1), 78-8 Skelding KA & Rostas JAP (2009) Neurochem Res 34, 1792-180 Dosemeci, A. et al (1994) J Biol Chem 269 (50), 31330-3133