Adrenocorticotropic hormone (ACTH), Rabbit Polyclonal Antibody

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Catalog Number
R-1491

    Product Info

  • Product Name Adrenocorticotropic hormone (ACTH), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Adrenocorticotropic hormone (ACTH) Polyclonal Antibody (Unconjugated), suitable for IHC-Paraffin-embedded.
  • Alternative Names Corticotropin; ACTH; Pro-opiomelanocortin; POMC;
  • Application(s) IHC-Paraffin-embedded
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human; mouse; rat; ACTH is highly conserved so cross-reactivity with other species is expected.
  • Species Reactivity Human, Mouse, Pig, Rat
  • Immunogen Description Porcine Adrenocorticotropic hormone (ACTH) conjugated to BSA
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Adrenocorticotropic hormone (ACTH) Polyclonal Antibody (Unconjugated), suitable for IHC-Paraffin-embedded.
  • Application(s) IHC-Paraffin-embedded
  • Application Details A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Adrenocorticotropic hormone (ACTH)
  • Specificity Human; mouse; rat; ACTH is highly conserved so cross-reactivity with other species is expected.
  • Target Host Species Pig
  • Species Reactivity Human, Mouse, Pig, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Porcine Adrenocorticotropic hormone (ACTH) conjugated to BSA
  • Sequence SYSMEHFRWGKPVGRKRRPVKVYP
  • Purity Description Affinity purified
  • Format Lyophilized with BSA
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Corticotropin; ACTH; Pro-opiomelanocortin; POMC;
  • Uniprot Number P01192
  • Uniprot Number/Name P01192 (COLI_PIG)
  • Scientific Background Adrenocorticotropic hormone (ACTH) is cleaved from the precursor pro-opiomelanocortin (POMC). The hormone is produced and secreted by the pituitary gland and stimulates release of cortisol by adrenal glands.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining in rat hypothalamus. 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to Adrenocorticotropic hormone (5 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).

    Citations & References