Endomorphin-1 and Endomorphin-2 (E-1/E-2), Rabbit Polyclonal Antibody

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Catalog Number
R-1495

    Product Info

  • Product Name Endomorphin-1 and Endomorphin-2 (E-1/E-2), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
  • Alternative Names EM 1; EM 2;
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human; mouse; rat. Endomorphin-1 is highly conserved molecule so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 = 70%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Antibodies to human Endomorphins 1 and 2 were made by immunising rabbits with the human Endomorphin-1 peptide (YPWF) and then selecting those antibodies that cross-reacted with both human Endomorphins 1 and 2.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Application Details A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Endomorphin-1 and Endomorphin-2 (E-1/E-2)
  • Specificity Human; mouse; rat. Endomorphin-1 is highly conserved molecule so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 = 70%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Antibodies to human Endomorphins 1 and 2 were made by immunising rabbits with the human Endomorphin-1 peptide (YPWF) and then selecting those antibodies that cross-reacted with both human Endomorphins 1 and 2.
  • Purity Description Affinity purified
  • Format Lyophilized with BSA
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names EM 1; EM 2;
  • Scientific Background Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining in rat ventral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to Endomorphin-1 and Endomorphin-2 (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).

    Citations & References