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Alkaline phosphatase (AP), Rabbit Polyclonal Antibody
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Catalog Number
R-1035
- Product Name Alkaline phosphatase (AP), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Alkaline phosphatase (AP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; ALPL;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Species Reactivity Human, Mouse, Rat
- Immunogen Description A synthetic peptide (ERSRCNTTQGNEVTS) corresponding to the amino acids 135-149 from human alkaline phosphatase. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Alkaline phosphatase (AP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human alkaline phosphatase has a predicted length of 524 residues and MW of 57 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Alkaline phosphatase (AP)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (ERSRCNTTQGNEVTS) corresponding to the amino acids 135-149 from human alkaline phosphatase. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; ALPL;
- Uniprot Number P05186
- Uniprot Number/Name P05186 (PPBT_HUMAN)
- Scientific Background Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules including nucleotides, proteins and alkaloids. There are at least four distinct groups of alkaline phosphatases: intestinal, placental, placental-like and tissue non-specific. The tissue non-specific form is a membrane bound glycosylated protein that is not expressed in any particular tissue.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
