SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
Species ReactivityHuman, Mouse, Rabbit, Rat
Immunogen DescriptionA synthetic peptide (PQQQPPYLHLAELTA) corresponding to a region (4-18) from human Calretinin. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human calretinin has a predicted length of 271 residues and MW of 32 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval by heat is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCalretinin (CR)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rabbit, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (PQQQPPYLHLAELTA) corresponding to a region (4-18) from human Calretinin. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsThe liquid formulation should be diluted in PBS (pH 7.4)
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCalretinin, also known as Calbindin 2, belongs to the calbindin family of proteins. It has six EF-hand domains which bind calcium.
Immunohistochemical staining with rabbit polyclonal antibody to human Calretinin (R-1042-100) in paraffin-embedded human mammary cancer sections at 1-2 µg/mL. Secondary biotinylated goat anti-rabbit IgG antibody at 10 µg/mL.
Western blot with rabbit polyclonal antibody to human Calretinin (R-1042-100) at 1-2 µg/mL in Hela cell lysate. Secondary HRP conjugated goat anti-rabbit IgG antibody at 1:3,000-10,000.