Calretinin (CR), Rabbit Polyclonal Antibody

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Catalog Number
R-1042

    Product Info

  • Product Name Calretinin (CR), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Calretinin (CR) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names CR; CALB2; CAB29; Calbindin 2;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
  • Species Reactivity Human, Mouse, Rabbit, Rat
  • Immunogen Description A synthetic peptide (PQQQPPYLHLAELTA) corresponding to a region (4-18) from human Calretinin. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Calretinin (CR) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human calretinin has a predicted length of 271 residues and MW of 32 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval by heat is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Calretinin (CR)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rabbit, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (PQQQPPYLHLAELTA) corresponding to a region (4-18) from human Calretinin. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
  • Purity Description Affinity purified on antigen column
  • Format Liquid (0.5 mL). 50% glycerol, 0.9 mg NaCl and 0.2 mg Na2HPO4
  • Reconstitution Instructions The liquid formulation should be diluted in PBS (pH 7.4)
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CR; CALB2; CAB29; Calbindin 2;
  • Uniprot Number P22676
  • Uniprot Number/Name P22676 (CALB2_HUMAN)
  • Scientific Background Calretinin, also known as Calbindin 2, belongs to the calbindin family of proteins. It has six EF-hand domains which bind calcium.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining with rabbit polyclonal antibody to human Calretinin (R-1042-100) in paraffin-embedded human mammary cancer sections at 1-2 µg/mL. Secondary biotinylated goat anti-rabbit IgG antibody at 10 µg/mL.

  • Western blot with rabbit polyclonal antibody to human Calretinin (R-1042-100) at 1-2 µg/mL in Hela cell lysate. Secondary HRP conjugated goat anti-rabbit IgG antibody at 1:3,000-10,000.

    Citations & References