SpecificityHuman; mouse; rat. Met-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 5.8%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionSynthetic human Met-enkephalin peptide (237-241) conjugated to BSA.
Application DetailsA dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMet-Enkephalin
SpecificityHuman; mouse; rat. Met-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 5.8%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionSynthetic human Met-enkephalin peptide (237-241) conjugated to BSA.
SequenceYGGFM
Purity DescriptionAffinity purified
FormatLyophilized with BSA
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 0.05 mL of sterile-filtered PBS (pH 7.4). Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundHuman Methionine enkephalin (Met-Enkephalin) is a small 5 amino acid peptide cleaved from the precursor pro-opiomelanocortin (POMC). Met-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Immunohistochemical staining in rat brainstem medulla. 4% paraformaldehyde fixed rat brain crystostat sections (10 µm) were incubated overnight at 4°C with Rabbit polyclonal antibody to human Met-Enkephalin (10 µg/mL) followed by incubation with donkey anti-rabbit Rhodamine Red conjugated secondary antibody (1:200).