Nitric Oxide Synthase 1 (NOS type 1), Rabbit Polyclonal Antibody

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Catalog Number
R-1323

    Product Info

  • Product Name Nitric Oxide Synthase 1 (NOS type 1), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Nitric Oxide Synthase 1 (NOS type 1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names neuronal NOS; nNOS; brain NOS; bNOS; NOS type 1; NOS1; NC-NOS; Constitutive NOS;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB in human and rat and by IHC in rat. Human; rat;
  • Species Reactivity Human, Rat
  • Immunogen Description A synthetic peptide (IAFIEESKKDTDEVFSS) corresponding to a sequence (1418-1434 aa) from human Nitric Oxide Synthase 1.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Nitric Oxide Synthase 1 (NOS type 1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human NOS (nNOS-1) has a predicted length of 1,434 residues and MW of 161 kDa. A concentration of 2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Nitric Oxide Synthase 1 (NOS type 1)
  • Specificity The specificity of this antibody has been confirmed by WB in human and rat and by IHC in rat. Human; rat;
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (IAFIEESKKDTDEVFSS) corresponding to a sequence (1418-1434 aa) from human Nitric Oxide Synthase 1.
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names neuronal NOS; nNOS; brain NOS; bNOS; NOS type 1; NOS1; NC-NOS; Constitutive NOS;
  • Uniprot Number P29475
  • Uniprot Number/Name P29475 (NOS1_HUMAN)
  • Scientific Background Nitric oxide synthase (NOS) catalyses the formation of nitric oxide, an important messenger molecule in functions such as homeostasis and synaptic plasticity. NOS is classified into three types: (1) neuronal NOS (nNOS) or brain NOS (bNOS) (2) inducible NOS (iNOS) or macrophage NOS (mNOS) and (3) endothelial NOS (eNOS).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot using Rabbit polyclonal antibody to human Nitric Oxide Synthase 1 (R-1323-100) at 1 µg/mL:

    Lane 1 and 2: Rat brain tissue lysate
    Lane 3: Rat medulla oblongata tissue lysate
    Lane 4: MCF-7 Whole cell lysate
    Lane 5: SMMC Whole cell lysate
    Lane 6: SW620 Whole cell lysate
    Lane 7: Colo320 Whole cell lysate

    Citations & References