Product NameGDNF family receptor alpha-1 (GFR alpha-1), Chicken Polyclonal Antibody
Product DescriptiongoogleChicken anti-GDNF family receptor alpha-1 (GFR alpha-1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Alternative NamesRET ligand 1;TGF-beta related neurotrophic factor receptor 1; GDNFR-alpha-1; GFR-alpha-1; TRNR1; RETL1; GFRA1;
Application(s)ICC, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificityHuman GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
Species ReactivityHuman
Immunogen DescriptionRecombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Application DetailsWB, ICC. A dilution of 1:5,000 to 1:10,000 is recommended for Western blot and 1:1,000 to 1:2,000 for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGDNF family receptor alpha-1 (GFR alpha-1)
SpecificityHuman GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionRecombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
SequenceUniprot P56159, whole molecule
Purity DescriptionAffinity purified
FormatConcentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
Reconstitution InstructionsStore antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally. Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
Storage InstructionsStore at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesRET ligand 1;TGF-beta related neurotrophic factor receptor 1; GDNFR-alpha-1; GFR-alpha-1; TRNR1; RETL1; GFRA1;
Scientific BackgroundGlial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFR_-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
Western-Blot detection of human GFRα-1, using Chicken polyclonal antibody to human GFRα-1. GFRα-1 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used.