SpecificityThis antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Species ReactivityCat, Dog, Human, Pig, Rabbit, Sheep
Immunogen DescriptionThe p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
ConjugateFITC
Purity DescriptionImmunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Application DetailsThis antibody is recommended for use in immunohistochemistry, immunofluorescence, and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/mL is recommended. Not appropriate for Western Blots or flow cytometry. For 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNGFR/p75 neurotrophin receptor (p75NTR)
SpecificityThis antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Target Host SpeciesHuman
Species ReactivityCat, Dog, Human, Pig, Rabbit, Sheep
Immunogen DescriptionThe p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Purity DescriptionImmunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
FormatLiquid PBS pH 7.2-7.6
Storage InstructionsThe antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
Batch NumberPlease see item label.
Expiration Date4 months after date of receipt (unopened vial)
Scientific BackgroundNerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.