NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (ATTO 488)

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Catalog Number
M-026-AT

    Product Info

  • Product Name NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (ATTO 488)
  • Product Description google Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (ATTO 488), suitable for ICC, IHC-Frozen, FC, IHC-Paraffin-embedded.
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Application(s) FC, ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR. Reacts with rat. Does not react with mouse or human NGFR
  • Species Reactivity Rat
  • Immunogen Description Rat p75NTR
  • Conjugate ATTO 488
  • Purity Description Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (ATTO 488), suitable for ICC, IHC-Frozen, FC, IHC-Paraffin-embedded.
  • Application(s) FC, ICC, IHC-Frozen, IHC-Paraffin-embedded
  • Application Details IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5µg/mL. Not suitable for western blots; not suitable for IH on formalin fixed tissues. FACS (20µg/mL) is recommended, unfixed cells.
  • Target NGFR/p75 neurotrophin receptor (p75NTR)
  • Specificity MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR. Reacts with rat. Does not react with mouse or human NGFR
  • Target Host Species Rat
  • Species Reactivity Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1
  • Clone Name MC192
  • Conjugate ATTO 488
  • Ex/Em Max Excitation maximum: 500 nm. Emission maximum: 520 nm.
  • Immunogen Description Rat p75NTR
  • Purity Description Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
  • Format Liquid
  • Storage Instructions The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
  • Batch Number Please see item label.
  • Expiration Date 4 months after date of receipt (unopened vial)
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Uniprot Number P07174
  • Uniprot Number/Name P07174 (TNR16_RAT)
  • Scientific Background Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.