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Proto-oncogene tyrosine-protein kinase receptor Ret (RET), Chicken Polyclonal Antibody
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Catalog Number
C-1663
- Product Name Proto-oncogene tyrosine-protein kinase receptor Ret (RET), Chicken Polyclonal Antibody
- Product Description Chicken anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
- Alternative Names Cadherin family member 12; Ret;
- Application(s) ICC, WB
- Antibody Host Chicken
- Antibody Type Polyclonal
- Specificity Human RET
- Species Reactivity Human
- Immunogen Description Recombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
- Conjugate Unconjugated
- Purity Description Affinity purified
- Regulatory Status For research use only.
Product Info
- Product Description Chicken anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
-
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- Application(s) ICC, WB
- Application Details WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:250 to 1:500 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Proto-oncogene tyrosine-protein kinase receptor Ret (RET)
- Specificity Human RET
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Chicken
- Antibody Type Polyclonal
- Antibody Isotype IgY
- Conjugate Unconjugated
- Immunogen Description Recombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
- Purity Description Affinity purified
- Format Liquid: Concentrated ammonium sulphate in PBS pH 7.4
- Reconstitution Instructions Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
- Storage Instructions Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Cadherin family member 12; Ret;
- Uniprot Number P07949
- Uniprot Number/Name P07949 (RET_HUMAN)
- Scientific Background The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
