Alternative NamesGDNF family receptor alpha-2; GFR-alpha-2; Neurturin receptor alpha; NTNR-alpha; NRTNR-alpha; TGF-beta-related neurotrophic factor receptor 2; GDNF receptor beta; GDNFR-beta; RET ligand 2
Application(s)IHC-Frozen, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityWestern blot analysis indicates a high level of specificity to GFR alpha-2 for this antiserum. This antibody is known to react with rat GFR alpha-2 and from IHC analysis, there appears to be no cross-reactivity with other members of the GFR family.
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide (PRVEKTPSLPDDLSD) as a part of human GFR alpha-2 protein (aa: 376-390) conjugated to KLH.
Application DetailsIHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains beautifully the large neurons in the rat DRG. This antibody also is ideal for WB analysis of rat DRG and brain homogenate. The optimal dilution should be determined by the end user, but a dilution of 1 in 500 is recommended for initial testing. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityWestern blot analysis indicates a high level of specificity to GFR alpha-2 for this antiserum. This antibody is known to react with rat GFR alpha-2 and from IHC analysis, there appears to be no cross-reactivity with other members of the GFR family.
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (PRVEKTPSLPDDLSD) as a part of human GFR alpha-2 protein (aa: 376-390) conjugated to KLH.
SequencePRVEKTPSLPDDLSD
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGDNF family receptor alpha-2; GFR-alpha-2; Neurturin receptor alpha; NTNR-alpha; NRTNR-alpha; TGF-beta-related neurotrophic factor receptor 2; GDNF receptor beta; GDNFR-beta; RET ligand 2
Scientific BackgroundFUNCTION: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.