1800 605-5127
+61 (0)8 8352 7711
Tyrosine Kinase Receptor B (TrkB), Rabbit Polyclonal Antibody
As low as
US$317.00
Only %1 left
Catalog Number
R-121
- Product Name Tyrosine Kinase Receptor B (TrkB), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2; Trkb
- Application(s) IHC-Frozen
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity Sequence was chosen to avoid cross reactivity with TrkA and TrkC. This antiserum recognises human, rat and mouse TrkB. Cross reactivity with other species has not yet been tested.
- Species Reactivity Human, Mouse, Rat
- Immunogen Description A synthetic peptide (AFPRLEPNSIDPEN(C)) as part of rat TrkB protein (aa: 54-67) conjugated to KLH
- Conjugate Unconjugated
- Purity Description Whole serum
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
-
Related Products
TrkB control peptide (54-67), Purified Peptide
- Application(s) IHC-Frozen
- Application Details IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. For WB, a 1:500 to 1:1000 dilution is recommended. The sequence of the immunogen and the conjugate are identical to the one used by Yan et al (see the Refs) and the antiserum appears to have similar characteristics. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Tyrosine Kinase Receptor B (TrkB)
- Specificity Sequence was chosen to avoid cross reactivity with TrkA and TrkC. This antiserum recognises human, rat and mouse TrkB. Cross reactivity with other species has not yet been tested.
- Target Host Species Rat
- Species Reactivity Human, Mouse, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype Mixed
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (AFPRLEPNSIDPEN(C)) as part of rat TrkB protein (aa: 54-67) conjugated to KLH
- Purity Description Whole serum
- Format Lyophilized
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2; Trkb
- Uniprot Number Q63604
- Uniprot Number/Name Q63604 (NTRK2_RAT)
- Scientific Background FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures (By similarity). Binds APS. Interacts with SQSTM1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons. PTM: Ligand-mediated auto-phosphorylation. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
-
Specific References
Warren PM et al. (2018) "Rapid and robust restoration of breathing long after spinal cord injury." Nat Commun. 2018 Nov. PMID: 30482901 9(1):4843. Application: IHC/IF. Species: Rat, spinal cord sections.
Wang T et al. (2016) "Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB." Nat Commun. 2016 Sep. PMID: 27687129 30(7):12976. Application: IF & structured illumination microscopy (SIM). Species: Rat, Mouse; fixed 4% PFA with 4% sucrose
Matusica D et al. (2016) Inhibition of motor neuron death in vitro and in vivo by a p75 neurotrophin receptor intracellular domain fragment. J Cell Sci. 2016 Feb 1;129(3):517-30. Application: WB.
Brock JH et al. (2010) Local and remote growth factor effects after primate spinal cord injury. J Neurosci. 2010 Jul 21;30(29):9728-37.
Gruber H. et al. (2008) Brain-derived neurotrophic factor and its receptor in the human and the sand rat intervertebral disc. Arthritis Res Ther. 2008; 10(4): R82. -
General References
Yan Q et al. J Comp Neurol 378:135-57 (1997).
Stoilov, P., et al., Biochem. Biophys. Res. Commun. 290(3):1054-1065 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Nakagawara, A., et al., Genomics 25(2):538-546 (1995).
Haniu, M., et al., Arch. Biochem. Biophys. 322(1):256-264 (1995).
Allen, S.J., et al., Neuroscience 60(3):825-834 (1994).
Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-8
Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90.
Nakajima K et al. Glia. 1998 Nov;24(3):272-8