Product NameTyrosine Kinase Receptor C (TrkC), Rabbit Polyclonal Antibody
Product DescriptiongoogleRabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for WB.
Alternative NamesGP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
Application(s)WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityHuman TrkC, and Mouse and rat TrkC. Species cross-reactivity determined by Western Blotting. No Cross reactivity by WB seen with TrkA or TrkB receptors.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionSynthetic peptide immunogen, QLWQEQGEAKLNSQN from the ECD of human TrkC, amino acids 172-186.
Application DetailsWestern Blotting (0.1 - 0.5 µg/mL): Tested on RIPA lysates from human and rodent tissue and cell lysates. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTyrosine Kinase Receptor C (TrkC)
SpecificityHuman TrkC, and Mouse and rat TrkC. Species cross-reactivity determined by Western Blotting. No Cross reactivity by WB seen with TrkA or TrkB receptors.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionSynthetic peptide immunogen, QLWQEQGEAKLNSQN from the ECD of human TrkC, amino acids 172-186.
SequenceQLWQEQGEAKLNSQN
Purity DescriptionAffinity purified
FormatLyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase
Scientific BackgroundReceptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Western blot analysis of TrkC expression in various human and rodent tissue and cell extracts (RIPA). Two prominent bands are detected at ~140 kDa and ~100 kDa, corresponding to TrkC full-length (FL) and truncated TrkC (T1) isoforms. Additional, uncharacterized lower molecular-weight bands are observed. SDS-PAGE: denatured and reduced; Transfer: Towbin's buffer (20% MeOH, no SDS); Membrane: PVDF (0.45 um); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: overnight at 2-8°C (1 µg/mL); Secondary antibody: anti-rabbit-HRP (1/10,000), 1 hours at RT; Detection: Chemiluminiscence.