Application DetailsWestern blot (WB) at a suggested dilution of 1:2,000 - 1:4,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunogen DescriptionRecombinant human CDNF protein produced using CHO-based cell line. Purified from cell culture supernatant.
Purity DescriptionPurified by protein G affinity chromatography
FormatPBS pH 7.4, with 0.1% sodium azide.
Storage InstructionsStore at -20°C or -70°C upon receipt. After opening maintain undiluted in smaller aliquots at -20°C or -70°C for up to 6 months. Avoid multiple freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCDNF (conserved dopamine neurotrophic factor) is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson's disease. When administered after 6-OHDA or MPTP -lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Western Blot testing of anti-CDNF antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.