SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Species ReactivityHuman, Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (188-210) from human FGF-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human FGF-2 (isoform 1) has a predicted length of 210 amino acids and MW of 23 kDa. A concentration of 1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetFibroblast growth factor 2 (FGF-2)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a region (188-210) from human FGF-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundFibroblast growth factors (FGFs) bind heparin and exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. FGF-2 has been implicated in diverse biological processes such as limb and nervous system development, wound healing and tumour growth. There are at least 3 isoforms of FGF-2 arising from alternative initiation.
Immunohistochemical staining of rabbit polyclonal antibody to human FGF-2 at 1-2 µg/ml in paraffin-embedded human mammary cancer sections. Biotinylated goat anti-rabbit IgG secondary antibody at 10 µg/ml.