Fibroblast growth factor 2 (FGF-2), Rabbit Polyclonal Antibody

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Catalog Number
R-1019
Discontinued

    Product Info

  • Product Name Fibroblast growth factor 2 (FGF-2), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Fibroblast growth factor 2 (FGF-2) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names Heparin-binding growth factor 2; HBGF-2; Basic fibroblast growth factor; FGFB; bFGF;Prostatropin; FGF2;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
  • Species Reactivity Human, Rat
  • Immunogen Description A synthetic peptide corresponding to a region (188-210) from human FGF-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Fibroblast growth factor 2 (FGF-2) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human FGF-2 (isoform 1) has a predicted length of 210 amino acids and MW of 23 kDa. A concentration of 1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Fibroblast growth factor 2 (FGF-2)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide corresponding to a region (188-210) from human FGF-2. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Heparin-binding growth factor 2; HBGF-2; Basic fibroblast growth factor; FGFB; bFGF;Prostatropin; FGF2;
  • Uniprot Number P09038
  • Uniprot Number/Name P09038 (FGF2_HUMAN)
  • Scientific Background Fibroblast growth factors (FGFs) bind heparin and exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. FGF-2 has been implicated in diverse biological processes such as limb and nervous system development, wound healing and tumour growth. There are at least 3 isoforms of FGF-2 arising from alternative initiation.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemical staining of rabbit polyclonal antibody to human FGF-2 at 1-2 µg/ml in paraffin-embedded human mammary cancer sections. Biotinylated goat anti-rabbit IgG secondary antibody at 10 µg/ml.

    Citations & References