pro-Nerve growth factor (proNGF), Sheep Polyclonal Antibody

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Catalog Number
S-080

    Product Info

  • Product Name pro-Nerve growth factor (proNGF), Sheep Polyclonal Antibody
  • Product Description google
    Sheep anti-pro-Nerve growth factor (proNGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
  • Alternative Names pro-brain nerve growth factor; proNGF; NGF;
  • Application(s) ELISA, IHC-Frozen, Neutralize, WB
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description The recombinant prodomain fragment of human nerve growth factor
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Sheep anti-pro-Nerve growth factor (proNGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
  • Application(s) ELISA, IHC-Frozen, Neutralize, WB
  • Application Details IHC, Immunofluorescence, ELISA, Western Blot, biological neutralization of proNGF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target pro-Nerve growth factor (proNGF)
  • Specificity The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description The recombinant prodomain fragment of human nerve growth factor
  • Purity Description Whole serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Maintain lyophilized antibody at 2-8°C for up to 12 months after date of receipt. After reconstitution keep undiluted aliquots at -20°C for up to 6 months for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names pro-brain nerve growth factor; proNGF; NGF;
  • Uniprot Number P01138
  • Uniprot Number/Name P01138 (NGF_HUMAN)
  • Scientific Background Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot of proNGF proteins produced in E.Coli. S-080-100 detects the human and mouse form of proNGF as well as the pro-domain peptide. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (1:1000 dilution); Secondary antibody: anti-sheep-HRP (1/3000), 2 hours at RT; Detection: Chemiluminiscence.

  • Immunofluorescence analysis of proNGF expression in human DU145 prostate cancer cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) DU145 cells were incubated with proNGF antibody S-080-100 (1:1000, red) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-sheep-PE antibody (1:100, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.


    The sheep anti-proNGF antibodies recognize recombinant proNGF, the prodomain of NGF and but not mouse mature NGF (50 ng in each lane).

    Citations & References

  • Specific References

    Makoudjou M et al. (2024) ProNGF processing in adult rat tissues and bioactivity of NGF pro domain peptides  FEBSPRESS Application: Rat Brain Tissue

    Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Neutralising (in-vivo).

    Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Western Blotting.

  • General References Srinivasan B et al. J Biol Chem. 2004 Oct 1;279(40):41839-4
    Yune TY et al. J Neurosci. 2007 Jul 18;27(29):7751-61.