PAP-inositol 1,4-phosphatase (PIP), Mouse Monoclonal Antibody

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Catalog Number
M-805

    Product Info

  • Product Name PAP-inositol 1,4-phosphatase (PIP), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-PAP-inositol 1,4-phosphatase (PIP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
  • Alternative Names 3 ' (2 '); 5 '-bisphosphate nucleotidase 1; bisophosphotase 3 '-nucleotidase 1; PAP-inositol-1;4-phosphotase; BPNT1
  • Application(s) ELISA, IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB against the recombinant protein and in HeLa cell lysate. This antibody has not yet been tested in other species.
  • Species Reactivity Human
  • Immunogen Description Recombinant protein of amino acids 1 to 100 of human PIP with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-PAP-inositol 1,4-phosphatase (PIP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
  • Application(s) ELISA, IHC-Frozen, WB
  • Application Details This antibody is recommended for WB, ELISA, sandwich ELISA, IHC and immunofluorescence. It is recommended to be used at 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. This antibody has a detection limit of 0.03ng/mL in sandwich ELISA when using the recombinant protein. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target PAP-inositol 1,4-phosphatase (PIP)
  • Specificity The specificity of this antibody has been confirmed by WB against the recombinant protein and in HeLa cell lysate. This antibody has not yet been tested in other species.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 2E1
  • Conjugate Unconjugated
  • Immunogen Description Recombinant protein of amino acids 1 to 100 of human PIP with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names 3 ' (2 '); 5 '-bisphosphate nucleotidase 1; bisophosphotase 3 '-nucleotidase 1; PAP-inositol-1;4-phosphotase; BPNT1
  • Uniprot Number O95861
  • Uniprot Number/Name O95861 (BPNT1_HUMAN)
  • Scientific Background
    PIP belongs to the inositol monophosphatase family. It hydrolyses adenosine 3'phosphate 5'-phosphosulfate to adenosine 5-phosphosulfate and 3'(2)'-phosphoadenosine 5'-phospate to AMP. It requires magnesium as a cofactor. Lithium is an uncompetive inhibitor and inositol 1,4-bisphosphate is a competitive inhibitor of PIP. Lithium is one of the drugs used to treat manic depression and the inhibition of PIP may account for lithium-induced nephrotoxicity.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunohistochemistry of mouse monoclonal antibody to human PIP [2E1], catalogue number M-805-100, at a concentration of 3 µg/mL, on formalin-fixed and paraffin-embedded human pancreas.

  • Immunofluorescence detection of PIP on HeLa cells when using mouse monoclonal antibody to human PIP [2E1], catalogue number M-805-100, at a concentration of 10 µg/mL.


    Western blot analysis of PIP in HeLa cell lysate using the mouse monoclonal antibody to human PIP [2E1], catalogue number M-085-100. This anitbody recognises a band of approximate molecular weight 37 KDa

    Citations & References