Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. The predicted length of human cathepsin D is 412 aa. The predicted MW is 34 kDa (active) and 52 kDa (precursor). A concentration of 1.0-2.0 µg/mL is recommended to detect cathepsin D in paraffin embedded tissues. Antigen retrieval by heat is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCathepsin D
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameCTD-19
ConjugateUnconjugated
Immunogen DescriptionHuman liver Cathepsin D
Purity DescriptionIgG
FormatLyophilized. Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA with 0.01 mg NaN3 as a preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage Instructions12 months after purchase at -20°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCathepsin D is an aspartyl protease expressed in all cells and has a role on the degradation of proteins in lysosomes. It is initially expressed as an inactive 52 kDa precursor and activated by proteolysis. This antibody detects both precursor and mature forms. It does not react with human cathepsins B, C, G and H and with bovine cathepsins D and B.