Caspase-3 (CASP-3), Rabbit Polyclonal Antibody

Only %1 left
Catalog Number
R-1823
Discontinued

    Product Info

  • Product Name Caspase-3 (CASP-3), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Caspase-3 (CASP-3) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, FC.
  • Alternative Names CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
  • Application(s) FC, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity This antibody is specific for caspase 3 as demonstrated by western blotting.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175) was used as the immunogen. Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Caspase-3 (CASP-3) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, FC.
  • Application(s) FC, IHC-Paraffin-embedded, WB
  • Application Details Western Blot : (0.1-0.5 µg/mL) tested in human hepatocarcinoma cell line SMMC, rat liver tissue, and rat thymus tissue. Immunohistochemistry : formalin fixed, paraffin embedded sections (0.5-1.0 µg/mL): with HIER; tested in human lung cancer tissue, rat intestine tissue, mouse intestine tissue,. Flow Cytometry (1-3 μg/10e6 cells): tested in human cell line K562. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Caspase-3 (CASP-3)
  • Specificity This antibody is specific for caspase 3 as demonstrated by western blotting.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175) was used as the immunogen. Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.
  • Purity Description Affinity purified
  • Format Lyophilized.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; CASP3; CPP32
  • Uniprot Number P42574
  • Uniprot Number/Name P42574 (CASP3_HUMAN)
  • Scientific Background Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A - Western blot analysis of Caspase 3 expression in rat liver (1), rat thymus (2) and SMCC whole cell lysate. Caspase-3 is detected at the expected molecular weight of 31 kDa. Samples were loaded at 50 µg/lane, and SDS-PAGE performed under reducing conditions. Transfer: nitrocellulose membrane. Blocking: 5% skim milk, 1.5 h at RT. Primary antibody: 0.5 µg/mL overnight at 2-8°C. Secondary antibody: Goat anti-rabbit IgG-HRP (1:10,000), 1.5 h at RT. Detection: ECL.
    B - Analysis of Caspase 3 expression in paraffin-embedded section of human lung cancer tissue by Immunohistochemistry. Heat-mediated antigen retrieval was performed in citrate buffer (pH6) for 20 minutes. The tissue section was blocked with 10% goat serum and then incubated with 1 µg/mL rabbit anti-caspase 3 antibody overnight at 2-8°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DAB as the chromogen.

  • C - Analysis of Caspase 3 expression in paraffin-embedded section of rat intestine tissue by Immunohistochemistry. Heat-mediated antigen retrieval was performed in citrate buffer (pH6) for 20 minutes. The tissue section was blocked with 10% goat serum and then incubated with 1 µg/mL rabbit anti-caspase 3 antibody overnight at 2-8°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DAB as the chromogen. D - Flow Cytometry analysis of caspase 3 expression (blue) in K562 cells. The cells were blocked with 10% normal goat serum, then incubated with primary antibody (1 µg/10^6 cells) for 30 minutes at 20C. Secondary antibody: fluorescent-labelled goat anti-rabbit (5-10 µg/10^6 cells), 30 minutes at 20C. Isotype control antibody (green) and unlabelled sample (red) were used as control conditions.

    Citations & References