Product DescriptionRabbit anti-Huntingtin-associated protein 1 (HAP-1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s)IHC-Frozen
Application DetailsIHC. A dilution of 1:500 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHuntingtin-associated protein 1 (HAP-1)
SpecificitySpecificity for HAP-1 was confirmed by IHC. This antiserum is known to react with rat HAP-1. Other species have not yet been tested.
Target Host SpeciesRat
Species ReactivityRat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionRecombinant rat HAP-1
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHuntingtin-associated protein 1; HAP1
Scientific BackgroundFUNCTION: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: In the brain, especially in the olfactory bulb and in the brain stem. No detectable expression in peripheral tissues such as lung, testis, spleen, and small intestine. SIMILARITY: Contains 1 HAP1 N-terminal domain.
IHC on rat DRG (free floating cryo section) using Rabbit antibody to recombinant rat HAP-1: whole serum (R-090-100) at a dilution of 1: 1000.
IHC on rat spinal cord (free floating cryo section) using Rabbit antibody to recombinant rat HAP-1: whole serum (R-090-100) at a dilution of 1: 1000. Motor neurons are intensely stained.
General ReferencesZhou, H. et al. (2003). J. Cell Biol. 163(1): 109-11 Fusco, F.R. et al. (2003). Eur. J. Neurosci. 18: 1- Pardo, R. et al. (2006). J. Neurosci. 26(5): 1635-164 Harper, S. Q. et al. (2005). Proc. Nat. Acad. Sci. (USA) 102: 5820-582 Qin, Z. et al. (2004). J. Neurosci. 24(1): 269-28 Tang, T. S. et al. (2003). Neuron 39(2): 227-23