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Saporin, Rabbit Polyclonal Antibody

As low as US$317.00
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Catalog Number
R-025
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    Product Info

  • Product Name Saporin, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Saporin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
  • Alternative Names Saponaria officinalis; Common soapwort
  • Application(s) ELISA, IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Confirmed to react with purified saporin. No cross-reactivity with other molecules has been reported.
  • Species Reactivity Plant
  • Immunogen Description Saporin, whole molecule
  • Conjugate Unconjugated
  • Purity Description Whole serum
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Saporin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
  • Application(s) ELISA, IHC-Frozen, WB
  • Application Details IHC, Immunofluorescence, ELISA, Western Blot. A dilution of 1:200 to 1: 2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Saporin
  • Specificity Confirmed to react with purified saporin. No cross-reactivity with other molecules has been reported.
  • Target Host Species Plant
  • Species Reactivity Plant
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype Mixed
  • Conjugate Unconjugated
  • Immunogen Description Saporin, whole molecule
  • Purity Description Whole serum
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Saponaria officinalis; Common soapwort
  • Uniprot Number P20656
  • Uniprot Number/Name P20656 (RIP6_SAPOF)
  • Scientific Background Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot analysis using rabbit polyclonal anti-saporin antisera (R-025-100). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).