Product DescriptionRabbit anti-Annexin I Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, ICC, FCM.
Application(s)FC, ICC, IHC-Paraffin-embedded, WB
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Annexin I (precursor) has a predicted length of 346 residues and a MW of 39 kDa. A concentration of 0.5-2.0 µg/mL is recommended to detect Annexin I in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAnnexin I
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (MVSEFLKQAWFIENEEQEYVQT) corresponding to a region (3-24) from the N-terminus of human Annexin I.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Immunohistochemical staining with Rabbit polyclonal antibody to Annexin I (R-1624-100) at 2 µg/mL in Human Mammary Cancer Tissue. Secondary biotin conjugated goat anti-rabbit IgG antibody at 10 µg/mL.