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Annexin V, Rabbit Polyclonal Antibody
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Catalog Number
R-1022
- Product Name Annexin V, Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Annexin V Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Annexin A5; Annexin 5; Lipocortin V; Endonexin II; Calphobindin I; PAP-I; PP4; VAC-alpha; Anchorin CII; CBP-I; ANX5; ENX2;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
- Species Reactivity Human, Mouse (Predicted), Rat
- Immunogen Description A synthetic peptide corresponding to a region (88-102 aa) from human Annexin V.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Annexin V Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Annexin V has a predicted length of 320 residues and a MW of 36 kDa. A concentration of 0.5-1 µg/mL is recommended to detect Annexin V in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Annexin V
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
- Target Host Species Human
- Species Reactivity Human, Mouse (Predicted), Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide corresponding to a region (88-102 aa) from human Annexin V.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal,0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Annexin A5; Annexin 5; Lipocortin V; Endonexin II; Calphobindin I; PAP-I; PP4; VAC-alpha; Anchorin CII; CBP-I; ANX5; ENX2;
- Uniprot Number P08758
- Uniprot Number/Name P08758 (ANXA5_HUMAN)
- Scientific Background Annexin V, also known as Annexin A5, belongs to the annexin family of calcium dependent phospholipid binding proteins. Annexin V has roles in cellular signal transduction, inflammation, growth and differentiation. Annexin V has been shown to have a high affinity for phosphatidylserine (PS) and is therefore a potential probe for the detection of apoptosis.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
Specifications
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Specific References
Wei X et al. (2016) "Fatty acid synthesis configures the plasma membrane for inflammation in diabetes." Nature. [Epub ahead of print]. Application: WB. Species: Mouse.