Intercellular adhesion molecule 1 (ICAM1), Rabbit Polyclonal Antibody

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Catalog Number
R-1724

    Product Info

  • Product Name Intercellular adhesion molecule 1 (ICAM1), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Intercellular adhesion molecule 1 (ICAM1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human
  • Species Reactivity Human
  • Immunogen Description E. coli-derived human ICAM1 recombinant protein (Position: L214-P532).Human ICAM1 shares 52% and 48% amino acid (aa) sequence identity with mouse and rat ICAM1, respectively.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Intercellular adhesion molecule 1 (ICAM1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human ICAM1 (precursor) has a predicted length of 532 residues and a MW of 58 kDa. A concentration of 0.5-1 µg/mL is recommended to detect ICAM1 in paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Intercellular adhesion molecule 1 (ICAM1)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description E. coli-derived human ICAM1 recombinant protein (Position: L214-P532).Human ICAM1 shares 52% and 48% amino acid (aa) sequence identity with mouse and rat ICAM1, respectively.
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names ICAM1; ICAM-1; Intercellular adhesion molecule 1; CD54;
  • Uniprot Number P05362
  • Uniprot Number/Name P05362 (ICAM1_HUMAN)
  • Scientific Background Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system. ICAM-1 is also expressed in all types of glial cells including astrocytes and microglia.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Rabbit anti-human iCAM 1 immunohistochemistry on paraffin sections of human intestinal cancer tissue. The antibody was used at a concentration of 1 µg/mL and an anti-rabbit secondary antibody was used at a concentration of 10 µg/mL.

    Citations & References