SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat; predicted to react with mouse and human due to sequence homology;
Species ReactivityHuman (Predicted), Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (SNKTRIDEANQRATK) corresponding to a region (187-201 aa) from the C-terminus of human Synaptosomal-associated protein 25 (SNAP25).
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Rat SNAP25 (isoform SNAP-25b) has a predicted length of 206 residues and MW of 23 kDa. A concentration of 1.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSynaptosomal-associated protein 25 (SNAP-25)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat; predicted to react with mouse and human due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman (Predicted), Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (SNKTRIDEANQRATK) corresponding to a region (187-201 aa) from the C-terminus of human Synaptosomal-associated protein 25 (SNAP25).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSNAP-25; Super protein; SUP; Synaptosomal-associated 25 kDa protein; Snap25; Snap;
Scientific BackgroundSNAP25 forms part of the SNARE core complex and associates with proteins involved in vesicle docking and membrane fusion (Ref: SWISSPROT).