Product DescriptionMouse anti-Immunoglobulin superfamily member 8 (CD316) Monoclonal Antibody (Unconjugated), suitable for ELISA.
Application(s)ELISA
Application DetailsThis antibody is recommended for sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetImmunoglobulin superfamily member 8 (CD316)
SpecificitySpecificity has been confirmed by ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name1E4
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human CD316 antigen [349-451], with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesImmunoglobulin superfamily member 8; CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; EWI-2; Keratinocytes-associated transmembrane protein 4; KCT-4; LIR-D1; IGSF8; CD81P3; EWI2; KCT4
CD316 antigen forms highly proximal, specific, and stoichiometric complexes with CD81 and CD9. It may play a key role in the diverse functions of CD81 and CD9 such as oocytes fertilisation or hepaptitis C virus function. It may regulate proliferation and differentiation of keratinocytes and may negatively regulate cell motility. It may also participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. CD316 antigen is a transmembrane protein that is expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. It is detected on a majority of B-cells, T-cells and natural killer cells but not on monocytes, polynuclear cells and platelets.
Sandwich ELISA using the anti-CD316 antigen monoclonal antibody as capture antibody. The detection limit for GST-tagged recombinant protein is 0.1 ng/ml.