Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetFucosyltransferase 10, alpha-(1,3)
SpecificitySpecificity has been confirmed by WB and ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name4H3
ConjugateUnconjugated
Immunogen DescriptionRecombinant human Fucosyltransferase 10, isoform 7 [1-92], with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAlpha-(1;3)-fucosyltransferase 10; Galactoside 3-L-fucosyltransferase 10; FucT-X; Fuc-TX; FUT10
Fucosyltransferase 10 is a probable fucosyltransferase that localises to the golgi apparatus, the Golgi stack membrane. It is a single-pass type II membrane protein. There are 7 named isoforms produced by alternative splicing.
Western blot detection of GST tagged recombinant human Fucosyltransferase 10. Note the GST tag alone is 26 kDa.
Sandwich ELISA using the anti-Fucosyltransferase 10 monoclonal antibody as capture antibody. The detection limit for GST-tagged recombinant protein is 1 ng/mL.