Semaphorin-4B (SEMA4B), Mouse Monoclonal Antibody

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Catalog Number
M-889

    Product Info

  • Product Name Semaphorin-4B (SEMA4B), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Semaphorin-4B (SEMA4B) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (Semaphorin) 4B
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Partial recombinant human SEMA4B (592-659) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Semaphorin-4B (SEMA4B) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Semaphorin-4B (SEMA4B)
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 4B2
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human SEMA4B (592-659) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (Semaphorin) 4B
  • Uniprot Number Q9NPR2
  • Uniprot Number/Name Q9NPR2 (SEM4B_HUMAN)
  • Scientific Background
    SEMA4B is a 837 amino acid polypeptide that has an estimated molecular weight of 92.7 kDa. It contains a Sema domain which occurs in semaphorins, which are a large family of secreted and transmembrane proteins, some of which function as repellent signals during axon guidance. It also contains a a PSI domain (plexin repeat). This is a cysteine rich repeat found in several different extracellular receptors.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of GST tagged recombinant human SEMA4B.

  • Sandwich ELISA using the anti-SEMA4B monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant SEMA4B is 0.1 ng/mL.

    Citations & References