Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSemaphorin-4B (SEMA4B)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name4B2
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human SEMA4B (592-659) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (Semaphorin) 4B
SEMA4B is a 837 amino acid polypeptide that has an estimated molecular weight of 92.7 kDa. It contains a Sema domain which occurs in semaphorins, which are a large family of secreted and transmembrane proteins, some of which function as repellent signals during axon guidance. It also contains a a PSI domain (plexin repeat). This is a cysteine rich repeat found in several different extracellular receptors.