Semaphorin-7A, Mouse Monoclonal Antibody

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Catalog Number
M-875

    Product Info

  • Product Name Semaphorin-7A, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Semaphorin-7A Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Semaphorin-L; Sema L; Semaphorin-K1; Sema K1; John-Milton-Hargen human blood group Ag; JMH blood group antigen; CD108 antigen; CDw108; SEMA7A; CD108; SEMAL
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Partial recombinant human Semaphorin-7A (amino acids 536-633) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Semaphorin-7A Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Semaphorin-7A
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 3D3
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human Semaphorin-7A (amino acids 536-633) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Semaphorin-L; Sema L; Semaphorin-K1; Sema K1; John-Milton-Hargen human blood group Ag; JMH blood group antigen; CD108 antigen; CDw108; SEMA7A; CD108; SEMAL
  • Uniprot Number O75326
  • Uniprot Number/Name O75326 (SEM7A_HUMAN)
  • Scientific Background
    Semaphorin-7A is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. It is an 80- kDa glycoprotein expressed on activated lymphocytes and erythrocytes. It may play an important role in the nervous system and in modulating immune function. It is expressed in placenta, testis, ovary, spleen, brain, spinal cord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of GST tagged recombinant human Semaphorin-7A. Note the GST tag alone is 26 kDa.

  • Sandwich ELISA using anti-Semaphorin-7A as a capture antibody. The detection limit for GST tagged recombinant Semaphorin 7A is 0.03 ng/mL.

    Citations & References