Semahorin-4D, Mouse Monoclonal Antibody

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Catalog Number
M-888

    Product Info

  • Product Name Semahorin-4D, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Semahorin-4D Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names BB18; A8; GR3; CD100 antigen; SEMA4D; CD100; SEMAJ
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Partial recombinant human Semaphorin-4D (115-224) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Semahorin-4D Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Semahorin-4D
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 3B4
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human Semaphorin-4D (115-224) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names BB18; A8; GR3; CD100 antigen; SEMA4D; CD100; SEMAJ
  • Uniprot Number Q92854
  • Uniprot Number/Name Q92854 (SEM4D_HUMAN)
  • Scientific Background
    Semaphorin-4D is an 863-amino acid polypeptide containing a secretory signal sequence, a single transmembrane domain, an immunoglobulin (Ig)-like domain, and a conserved 500-amino acid sema domain. It was first identified as a cell surface protein of resting T cells and was the first semaphorin believed to be involved in the immune system. In addition to playing a functional role in the immune system it may also act in the nervous system. It induces B-cells to aggregate and improves their viability in vitro. It is strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen and thymus. It is expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon or liver.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of human Semaphorin-4D expression in human promyelocytic leukemia whole cell lysate.

  • Sandwich ELISA using the anti-Semaphorin-4D monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant Semaphorin-4D is 0.3 ng/mL.

    Citations & References