Product DescriptionMouse anti-Sodium channel protein type 8 subunit alpha Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB, immunofluorescence and direct ELISA. The recommended dilution for this antibody is 10 µg/mL for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSodium channel protein type 8 subunit alpha
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human, mouse and rat. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody IsotypeIgG2a Kappa
Immunogen DescriptionPartial recombinant human Sodium channel protein type 8 subunit alpha (amino acids 1854-1951) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS (pH 7.2 - 7.6)
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSodium channel protein type VIII subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.6; SCN8A; MED
Sodium channel protein type 8 subunit alpha mediates the voltage-dependent sodium ion permeability of excitable membranes. Adopting opened or closed conformation in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na ions may pass in accordance with their electrochemical gradient. It is a multi-pass transmembrane protein that is widely expressed in neurons of the central and peripheral nervous system. There are four named isoforms. The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment, S4. Segments S4 are thought to be the voltage-sensors and are characterised by a series of positively charged amino acids at every third position.
Immunofluorescent detection of Sodium channel protein type 8 subunit alpha expression in mouse NIH/ Swiss embryo cell lysate. The anti-Sodium channel protein type 8 subunit alpha primary antibody, catalogue number M-864-100, was used at a concentration of 10 µg/mL.