Application DetailsWestern Blotting (WB). A concentration of 0.2-1.0 µg/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL of sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesFSH-R; FSHR; Follitropin receptor; LGR1;
Scientific BackgroundFollicle-stimulating hormone receptor (FSHR) is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. FSHR is a receptor for follicle stimulating hormone. At least 2 isoforms arising from alternative splicing have been described. Activates adenylate cyclase and induces cAMP production through the activation of PI3K-AKT and SRC-ERK1/2 signaling pathways.
Western blot analysis of FSH Receptor expression in rat testis extract (lane 1), rat ovary extract (lane 2), mouse testis extract (lane 3) mouse ovary extract (lane 4) and HELA whole cell lysates (lane 5). Rabbit antibody to FSH Receptor (0.5 µg/mL) detects a clear, strong band at 78 kDa corresponding to the MW of FSHR.