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Connexin-43 (Cx43), Rabbit Polyclonal Antibody
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Catalog Number
R-1607
- Product Name Connexin-43 (Cx43), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Connexin-43 (Cx43) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Cx43; gap junction alpha-1 protein; GJA1; GJAL; Gap junction 43 kDa heart protein;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity Human Cxn 43, no cross reactivity to other family members is expected
- Species Reactivity Human, Mouse, Rat
- Immunogen Description A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Connexin-43 (Cx43) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Connexin-43 is 382 residues and MW of 43 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Connexin-43 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Connexin-43 (Cx43)
- Specificity Human Cxn 43, no cross reactivity to other family members is expected
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Cx43; gap junction alpha-1 protein; GJA1; GJAL; Gap junction 43 kDa heart protein;
- Uniprot Number P17302
- Uniprot Number/Name P17302 (CXA1_HUMAN)
- Scientific Background A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighbouring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-43, also known as Gap junction alpha-1 (GJA1), belongs to the class II (alpha) group of connexins and is expressed in the heart and foetal cochlea.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.