Connexin-43 (Cx43), Rabbit Polyclonal Antibody

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Catalog Number
R-1607

    Product Info

  • Product Name Connexin-43 (Cx43), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Connexin-43 (Cx43) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names Cx43; gap junction alpha-1 protein; GJA1; GJAL; Gap junction 43 kDa heart protein;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Human Cxn 43, no cross reactivity to other family members is expected
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Connexin-43 (Cx43) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Connexin-43 is 382 residues and MW of 43 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Connexin-43 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Connexin-43 (Cx43)
  • Specificity Human Cxn 43, no cross reactivity to other family members is expected
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide corresponding to the amino acids 366-382 from the C-terminus of human Connexin-43.
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Cx43; gap junction alpha-1 protein; GJA1; GJAL; Gap junction 43 kDa heart protein;
  • Uniprot Number P17302
  • Uniprot Number/Name P17302 (CXA1_HUMAN)
  • Scientific Background A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighbouring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-43, also known as Gap junction alpha-1 (GJA1), belongs to the class II (alpha) group of connexins and is expressed in the heart and foetal cochlea.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

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