Alpha-2A adrenergic receptor (Alpha-2AAR), Rabbit Polyclonal Antibody

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Catalog Number
R-1034

    Product Info

  • Product Name Alpha-2A adrenergic receptor (Alpha-2AAR), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Alpha-2A adrenergic receptor (Alpha-2AAR) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names ADRA2R; ADRAR; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2 adrenergic receptor subtype C10; ADRA2A;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide (FGYCNSSLNPVIYTIFNH) corresponding to amino acids 414-431 from human Adrenergic Receptor alpha 2a. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Alpha-2A adrenergic receptor (Alpha-2AAR) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Adrenergic Receptor alpha 2a has a predicted length of 450 amino acids and MW of 49 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Alpha-2A adrenergic receptor (Alpha-2AAR)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (FGYCNSSLNPVIYTIFNH) corresponding to amino acids 414-431 from human Adrenergic Receptor alpha 2a. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names ADRA2R; ADRAR; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2 adrenergic receptor subtype C10; ADRA2A;
  • Uniprot Number P08913
  • Uniprot Number/Name P08913 (ADA2A_HUMAN)
  • Scientific Background Alpha 2a Adrenergic Receptors (ADRA2A) are multi-pass membrane proteins that belong to the G-protein coupled receptor 1 family. Their function is to mediate the inhibition of adenylate cyclase through the action of G-proteins.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot using rabbit polyclonal antibody to human alpha 2a Adrenergic Receptor (R-1034-100) at 1 µg/mL.
    Secondary HRP conjugated goat anti-rabbit IgG antibody at 1:3,000-10,000.

    Lane 1: Rat testicular tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: MCF7 whole cell lysate
    Lane 4: MM453 whole cell lysate
    Lane 5: SMMC whole cell lysate
    Lane 6: Hela whole cell lysate
    Lane 7: Colo320 whole cell tissue lysate

    Citations & References