SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB, IHC); rat (WB); predicted to react with mouse due to sequence homology;
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide (SGDYDSMKEPCFREEN) corresponding to a region (18-33) from human C-X-C Chemokine receptor 4. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human CXCR4 (isoform 1) has a predicted length of 352 residues and MW of 40 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in human formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval (Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins) is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetC-X-C Chemokine receptor 4 (CXCR4)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB, IHC); rat (WB); predicted to react with mouse due to sequence homology;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (SGDYDSMKEPCFREEN) corresponding to a region (18-33) from human C-X-C Chemokine receptor 4. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to six months. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe C-X-C Chemokine receptor 4 (CXCR4) is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. CXCR4 is a specific receptor for stromal cell-derived factor-1 (SDF-1) and is widely expressed in tissues including peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla. At least 2 isoforms are produced by alternative splicing.