Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human RAGE (isoform 1) has a predicted length of 404 residues and MW of 43 kDa. A concentration of 1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetReceptor for advanced glycosylation end products (RAGE)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB, IHC), Rat (IHC). Predicted to react with mouse due to sequence homology
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (178-196 aa) from human RAGE.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe Receptor for Advanced Glycation Endproducts (RAGE) is also known as the Advanced glycosylation end product-specific receptor (AGER). RAGE is expressed in endothelial cells and interacts with many molecules including advanced glycosylation end products (AGE) and amyloid beta peptide. At least 2 RAGE isoforms are produced by alternative splicing. Isoform 1 is a single-pass type I membrane protein whereas isoform 2 is secreted.