N-methyl-D-aspartate receptor 2B (NMDAR2B), Rabbit Polyclonal Antibody

Product Info

Product Name N-methyl-D-aspartate receptor 2B (NMDAR2B), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-N-methyl-D-aspartate receptor 2B (NMDAR2B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Alternative Names GRIN2B; NR2B; NMDAR2B; NR3;
Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat; Mouse; Rabbit;
Species Reactivity Human, Mouse, Rabbit, Rat
Immunogen Description A synthetic peptide corresponding to a region (1131-1146 aa) from human N-methyl-D-aspartate receptor 2B.
Conjugate Unconjugated
Purity Description Affinity purified on antigen column
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-N-methyl-D-aspartate receptor 2B (NMDAR2B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
Application Details Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. A dilution of 1:1,000 is suggested for IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target N-methyl-D-aspartate receptor 2B (NMDAR2B)
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat; Mouse; Rabbit;
Target Host Species Human
Species Reactivity Human, Mouse, Rabbit, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide corresponding to a region (1131-1146 aa) from human N-methyl-D-aspartate receptor 2B.
Purity Description Affinity purified on antigen column
Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names GRIN2B; NR2B; NMDAR2B; NR3;
Uniprot Number Q00960
Uniprot Number/Name Q00960 (NMDE2_RAT)
Scientific Background N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channels are formed from heteromers composed of three different subunits: the key receptor zeta subunit NMDAR1 (GRIN1) with an NMDAR2 epsilon subunit: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), or NMDAR2D (GRIN2D) and a third subunit: GRIN3A or GRIN3B.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Staining of rabbit retina with antibody R-1563-100. NMDAR 2B immunoreactivity (red) was confined in the entire plexus of the IPL. Ocassionally there were labelled blood vessels. Cryostat sections of rabbit eyecaps were incubated overnight in NMDAR 2B antibody (1:1,000) at RT. Donkey anti-rabbit Cy3 secondary (1:1,000) was used. (INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer). Photo courtesy of Alejandro Vila, Univ of Texas Health Science Center, Houston, Texas.

Citations & References

Specific References Moore KB et al. (2020), Localized Calcium Signaling and the Control of Coupling at Cx36 Gap Junctions. eNeuro. 7(2). Application: ICC/IF.