Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. A concentration of 0.5-1.0 µg/mL is recommended to detect NMDAR2A in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetN-methyl-D-aspartate receptor 2A (GluN2A)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat (WB, IHC); Mouse (WB). Expected to react with human.
Target Host SpeciesHuman
Species ReactivityHuman (Predicted), Mouse, Rat
Immunogen DescriptionA synthetic peptide corresponding to a region (1360-1376 aa) from human N-methyl-D-aspartate receptor 2A.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channels are formed from heteromers composed of three different subunits: the key receptor zeta subunit NMDAR1 (GRIN1) with an NMDAR2 epsilon subunit: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), or NMDAR2D (GRIN2D) and a third subunit: GRIN3A or GRIN3B.