Alternative NamesGENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
Application(s)WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThe specificity of this antibody has been confirmed by WB. Human and rat. The antibody is also expected to work on mouse because of protein homology.
Species ReactivityHuman, Mouse (Predicted), Rat
Immunogen DescriptionA synthetic peptide corresponding to a sequence in the middle region of human ATPase subunit F6 (ATP5J).
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsWestern blot, 0.1-0.5 µg/mL, cell lysates. Predicted size: 12-15 kDa , reduced conditions. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB. Human and rat. The antibody is also expected to work on mouse because of protein homology.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a sequence in the middle region of human ATPase subunit F6 (ATP5J).
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C up to six months. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
Scientific BackgroundMitochondrial membrane ATP synthase (F1F0 ATPsynthase or Complex V) produces ATP from ADP in the presence of a protongradient across the membrane which is generated by electron transport complexesof the respiratory chain. F-type ATPases consist of two structural domains, F1 -containing the extramembraneous catalytic core and F0 -containing the membrane proton channel, linked together by a central stalk anda peripheral stalk. During catalysis, ATP synthesis in the catalytic domain ofF1 is coupled via a rotary mechanism of the central stalk subunitsto proton translocation. Part of the complex F0 domain and theperipheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplexand subunit a/ATP6 static relative to the rotary elements. Also involved in therestoration of oligomycin-sensitive ATPase activity to depleted F1-F0complexes. (Uniprot: P18859).