Application DetailsWestern blot, 0.1-0.5 µg/mL, cell lysates. Predicted size: 12-15 kDa , reduced conditions. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C up to six months. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
Scientific BackgroundMitochondrial membrane ATP synthase (F1F0 ATPsynthase or Complex V) produces ATP from ADP in the presence of a protongradient across the membrane which is generated by electron transport complexesof the respiratory chain. F-type ATPases consist of two structural domains, F1 -containing the extramembraneous catalytic core and F0 -containing the membrane proton channel, linked together by a central stalk anda peripheral stalk. During catalysis, ATP synthesis in the catalytic domain ofF1 is coupled via a rotary mechanism of the central stalk subunitsto proton translocation. Part of the complex F0 domain and theperipheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplexand subunit a/ATP6 static relative to the rotary elements. Also involved in therestoration of oligomycin-sensitive ATPase activity to depleted F1-F0complexes. (Uniprot: P18859).