ATP synthase-coupling factor 6, mitochondrial (ATPase subunit F6), Rabbit Polyclonal Antibody

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Catalog Number
R-1766

    Product Info

  • Product Name ATP synthase-coupling factor 6, mitochondrial (ATPase subunit F6), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-ATP synthase-coupling factor 6, mitochondrial (ATPase subunit F6) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names GENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB. Human and rat. The antibody is also expected to work on mouse because of protein homology.
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide corresponding to a sequence in the middle region of human ATPase subunit F6 (ATP5J).
  • Conjugate Unconjugated
  • Purity Description Affinity purified on antigen column
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-ATP synthase-coupling factor 6, mitochondrial (ATPase subunit F6) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western blot, 0.1-0.5 µg/mL, cell lysates. Predicted size: 12-15 kDa , reduced conditions. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target ATP synthase-coupling factor 6, mitochondrial (ATPase subunit F6)
  • Specificity The specificity of this antibody has been confirmed by WB. Human and rat. The antibody is also expected to work on mouse because of protein homology.
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide corresponding to a sequence in the middle region of human ATPase subunit F6 (ATP5J).
  • Purity Description Affinity purified on antigen column
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C up to six months. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names GENE NAME: ATP5J; ATP5A; ATPM | PROTEIN NAMES: ATPase subunit F6;
  • Uniprot Number P18859
  • Uniprot Number/Name P18859 (ATP5J_HUMAN)
  • Scientific Background Mitochondrial membrane ATP synthase (F1F0 ATPsynthase or Complex V) produces ATP from ADP in the presence of a protongradient across the membrane which is generated by electron transport complexesof the respiratory chain. F-type ATPases consist of two structural domains, F1 -containing the extramembraneous catalytic core and F0 -containing the membrane proton channel, linked together by a central stalk anda peripheral stalk. During catalysis, ATP synthesis in the catalytic domain ofF1 is coupled via a rotary mechanism of the central stalk subunitsto proton translocation. Part of the complex F0 domain and theperipheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplexand subunit a/ATP6 static relative to the rotary elements. Also involved in therestoration of oligomycin-sensitive ATPase activity to depleted F1-F0complexes. (Uniprot: P18859).
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

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