Alternative NamesSARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
Application(s)ELISA, WB
Antibody HostRabbit
Antibody TypePolyclonal
Specificity Predicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
Species ReactivityVirus
Immunogen DescriptionAnti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (R-1967-100) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
ConjugateUnconjugated
Concentration1 mg/mL
Purity DescriptionAffinity-purified via peptide column.
SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be used for the detection of SARS-CoV-2 (COVID-19) Nucleocapsid protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
TargetSARS-CoV-2 nucleocapsid phosphoprotein
Specificity Predicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
Target Host SpeciesVirus
Species ReactivityVirus
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionAnti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (R-1967-100) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
HomologyPredicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
Isoform InformationSARS-CoV-2 (COVID-19) Nucleocapsid has one isoform (419aa).
Purity DescriptionAffinity-purified via peptide column.
FormatLiquid. SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide. Conc.1 mg/mL
Concentration1 mg/mL
Reconstitution InstructionsSpin vial briefly before opening.
Storage InstructionsSARS-CoV-2 (COVID-19) Nucleocapsid antibody can be stored at 2-8°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
NCBI Official Full NameNucleocapsid Phosphoprotein
NCBI Official SymbolN
NCBI OrganismSARS-CoV-2
Scientific BackgroundCoronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).
Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, 10-306. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, R-1967-100, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.
Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, R-1967-100, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL
Figure 3 ELISA Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid antibody, R-1967-100 (1 μg/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.