SARS-CoV-2 nucleocapsid phosphoprotein, Rabbit Polyclonal Antibody

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Catalog Number
R-1967

    Product Info

  • Product Name SARS-CoV-2 nucleocapsid phosphoprotein, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-SARS-CoV-2 nucleocapsid phosphoprotein Polyclonal Antibody (Unconjugated), suitable for ELISA, WB.
  • Alternative Names SARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
  • Application(s) ELISA, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity Predicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
  • Species Reactivity Virus
  • Immunogen Description Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (R-1967-100) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein.

    The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
  • Conjugate Unconjugated
  • Concentration 1 mg/mL
  • Purity Description Affinity-purified via peptide column.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-SARS-CoV-2 nucleocapsid phosphoprotein Polyclonal Antibody (Unconjugated), suitable for ELISA, WB.
  • Application(s) ELISA, WB
  • Application Details WB: 0.5-2 μg/mL

    SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be used for the detection of SARS-CoV-2 (COVID-19) Nucleocapsid protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
  • Target SARS-CoV-2 nucleocapsid phosphoprotein
  • Specificity Predicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
  • Target Host Species Virus
  • Species Reactivity Virus
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (R-1967-100) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein.

    The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
  • Homology Predicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
  • Isoform Information SARS-CoV-2 (COVID-19) Nucleocapsid has one isoform (419aa).
  • Purity Description Affinity-purified via peptide column.
  • Format Liquid. SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide. Conc.1 mg/mL
  • Concentration 1 mg/mL
  • Reconstitution Instructions Spin vial briefly before opening.
  • Storage Instructions SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be stored at 2-8°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names SARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
  • Uniprot Number P0DTC9
  • Uniprot Number/Name P0DTC9 (NCAP_SARS2)
  • NCBI Official Full Name Nucleocapsid Phosphoprotein
  • NCBI Official Symbol N
  • NCBI Organism SARS-CoV-2
  • Scientific Background Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).
  • Research Area Infectious Disease
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein
    Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, 10-306. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, R-1967-100, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

  • Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein
    Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, R-1967-100, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL


    Figure 3 ELISA Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein
    Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid antibody, R-1967-100 (1 μg/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.

    Citations & References

  • General References Zhu et al. N Engl J Med. 2020 Feb 20;382(8):727-733.
    Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575.
    Chang et al. J Biomed Sci. 2006 Jan;13(1):59-72.
    Cui et al. J Virol. 2015 Sep;89(17):9029-43.
    Chen et al. Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53.
    Emerg Infect Dis. 2004 Sep;10(9):1558-62.