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SARS-CoV spike protein, Rabbit Polyclonal Antibody

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Catalog Number

R-1943

Product Info

Product Name SARS-CoV spike protein, Rabbit Polyclonal Antibody
Product Description google Rabbit anti-SARS-CoV spike protein Polyclonal Antibody (Unconjugated), suitable for ELISA.
Alternative Names SARS-CoV Spike Antibody: E2, Spike glycoprotein, E2, S glycoprotein
Application(s) ELISA
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 56%, homology 69%)
Species Reactivity Virus
Immunogen Description Anti-SARS-CoV Spike antibody (R-1943-100) was raised against a peptide corresponding to 16 amino acids near the center of SARS-CoV Spike glycoprotein.

The immunogen is located within amino acids 410-460 of SARS-CoV Spike.
Conjugate Unconjugated
Concentration 1 mg/mL
Purity Description Affinity-purified via peptide column.
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-SARS-CoV spike protein Polyclonal Antibody (Unconjugated), suitable for ELISA.
Application(s) ELISA
Application Details SARS-CoV Spike antibody can be used for the detection of SARS-CoV Spike protein in ELISA. It will detect 2 ng of free peptide at 1 μg/mL.
Target SARS-CoV spike protein
Specificity Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 56%, homology 69%)
Target Host Species Virus
Species Reactivity Virus
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description Anti-SARS-CoV Spike antibody (R-1943-100) was raised against a peptide corresponding to 16 amino acids near the center of SARS-CoV Spike glycoprotein.

The immunogen is located within amino acids 410-460 of SARS-CoV Spike.
Homology Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 56%, homology 69%)
Isoform Information SARS-CoV Spike has one isoform (1255aa).
Purity Description Affinity-purified via peptide column.
Format Liquid. SARS-CoV Spike Antibody is supplied in PBS containing 0.02% sodium azide. Conc.1 mg/mL
Concentration 1 mg/mL
Reconstitution Instructions Spin vial briefly before opening.
Storage Instructions SARS-CoV Spike antibody can be stored at 2-8°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names SARS-CoV Spike Antibody: E2, Spike glycoprotein, E2, S glycoprotein
Uniprot Number P59594
Uniprot Number/Name P59594 (SPIKE_SARS)
NCBI Official Full Name Spike glycoprotein
NCBI Official Symbol S
NCBI Organism SARS coronavirus
Scientific Background SARS-CoV Spike Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
Research Area Infectious Disease
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Figure 1 Western Blot Validation with Human Recombinant Protein
Loading: 50 ng per lane of human SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Spike R-1943-100 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 ELISA Test
Antibodies: SARS-CoV Spike Antibody, R-1943-100 (1 μg/mL). A direct ELISA was performed using immunogen as coating antigen and the anti-SARS-CoV Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000ng/mL.

Citations & References

General References Marra et al. Science 2003;300:1399-404.
Rota et al. Science 2003;300:1394-9.
Navas-Nartin et al. J Neurovirol. 2004;10:75-85.
Li et al. Nature 2003;426:450-4.