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GDNF family receptor alpha-1 (GFR alpha-1), Chicken Polyclonal Antibody
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US$437.00
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Catalog Number
C-1659
- Product Name GDNF family receptor alpha-1 (GFR alpha-1), Chicken Polyclonal Antibody
- Product Description Chicken anti-GDNF family receptor alpha-1 (GFR alpha-1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
- Alternative Names RET ligand 1;TGF-beta related neurotrophic factor receptor 1; GDNFR-alpha-1; GFR-alpha-1; TRNR1; RETL1; GFRA1;
- Application(s) ICC, WB
- Antibody Host Chicken
- Antibody Type Polyclonal
- Specificity Human GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
- Species Reactivity Human
- Immunogen Description Recombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
- Conjugate Unconjugated
- Purity Description Affinity purified
- Regulatory Status For research use only.
Product Info
- Product Description Chicken anti-GDNF family receptor alpha-1 (GFR alpha-1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
-
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- Application(s) ICC, WB
- Application Details WB, ICC. A dilution of 1:5,000 to 1:10,000 is recommended for Western blot and 1:1,000 to 1:2,000 for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target GDNF family receptor alpha-1 (GFR alpha-1)
- Specificity Human GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Chicken
- Antibody Type Polyclonal
- Antibody Isotype IgY
- Conjugate Unconjugated
- Immunogen Description Recombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
- Sequence Uniprot P56159, whole molecule
- Purity Description Affinity purified
- Format Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
-
Reconstitution Instructions
Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life. - Storage Instructions Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names RET ligand 1;TGF-beta related neurotrophic factor receptor 1; GDNFR-alpha-1; GFR-alpha-1; TRNR1; RETL1; GFRA1;
- Uniprot Number P56159
- Uniprot Number/Name P56159 (GFRA1_HUMAN)
- Scientific Background Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFR_-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
