GDNF family receptor alpha-3 (GFR alpha-3), Chicken Polyclonal Antibody

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Catalog Number
C-1661

    Product Info

  • Product Name GDNF family receptor alpha-3 (GFR alpha-3), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-GDNF family receptor alpha-3 (GFR alpha-3) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GFRA3;
  • Application(s) ICC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Human GFRa-3. No cross-reactivity with hGFRa-1, hGFRa-2, hGFRa-4.
  • Species Reactivity Human
  • Immunogen Description Recombinant His-tagged human GFRa-3 protein produced using CHO-based cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-GDNF family receptor alpha-3 (GFR alpha-3) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
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  • Application(s) ICC, WB
  • Application Details WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:1500 to1:3000 for immunocytochemistry. Biosensis recommends that optimal dilutions/concentrations should be determined by the end user.
  • Target GDNF family receptor alpha-3 (GFR alpha-3)
  • Specificity Human GFRa-3. No cross-reactivity with hGFRa-1, hGFRa-2, hGFRa-4.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Recombinant His-tagged human GFRa-3 protein produced using CHO-based cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
  • Sequence Uniprot O60609
  • Purity Description Affinity purified
  • Format Concentrated ammonium sulphate in PBS pH 7.4 contain no preservatives
  • Reconstitution Instructions Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
    Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life.
  • Storage Instructions Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GFRA3;
  • Uniprot Number O60609
  • Uniprot Number/Name O60609 (GFRA3_HUMAN)
  • Scientific Background The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used.

    Citations & References