Product NameGDNF family receptor alpha-4 (GFR alpha-4), Chicken Polyclonal Antibody
Product DescriptiongoogleChicken anti-GDNF family receptor alpha-4 (GFR alpha-4) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative NamesGDNF receptor alpha-4; Persephin receptor; GDNFR-alpha-4; GFR-alpha-4;
Application(s)IHC-Frozen, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificityHuman GFRa-4. This antibody does not cross-react with hGFRa-1, hGFRa-2, hGFRa-3
Species ReactivityHuman
Immunogen DescriptionRecombinant His-tagged human GFRa-4 protein produced using CHO-based cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Application DetailsWB, IHC. A dilution of 1:5 000-1:10 000 is recommended for Western blots and 1:1 000 to 1:3 000 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGDNF family receptor alpha-4 (GFR alpha-4)
SpecificityHuman GFRa-4. This antibody does not cross-react with hGFRa-1, hGFRa-2, hGFRa-3
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionRecombinant His-tagged human GFRa-4 protein produced using CHO-based cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
SequenceUniprot Q9GZZ7
Purity DescriptionAffinity purified
FormatLiquid in PBS pH 7.4 containing no preservatives
Reconstitution Instructionsready to dilute once thawed
Storage InstructionsStore at -80°C upon receipt. Divide antibody into aliquots upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesGDNF receptor alpha-4; Persephin receptor; GDNFR-alpha-4; GFR-alpha-4;
Scientific BackgroundGFR_-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor.
Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. GFRα-4 was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Line 2. Prestained protein ladder (Naxo 8003).