NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (FITC)

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Catalog Number
M-1819-FT

    Product Info

  • Product Name NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (FITC)
  • Product Description google Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for FC, ICC.
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Application(s) FC, ICC
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
  • Species Reactivity Human, Mouse, Rat
  • Immunogen Description Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
  • Conjugate FITC
  • Purity Description Protein A purified IgG was labelled with FITC and free dye removed by gel filtration.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for FC, ICC.
  • Related Products NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody
    NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (ATTO 488)
  • Application(s) FC, ICC
  • Application Details Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation.
    Flow Cytometry: 5-20 µg/mL.
    Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target NGFR/p75 neurotrophin receptor (p75NTR)
  • Specificity Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG2a
  • Clone Name 8J2
  • Conjugate FITC
  • Ex/Em Max Excitation maximum: 490 nm. Emission maximum: 525 nm.
  • Immunogen Description Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
  • Purity Description Protein A purified IgG was labelled with FITC and free dye removed by gel filtration.
  • Format Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
  • Storage Instructions Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 4 months after date of receipt (unopened vial)
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Uniprot Number P08138
  • Uniprot Number/Name P08138 (TNR16_HUMAN)
  • Scientific Background p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Live cell imaging of NGFR/p75 receptor in human SH-SY5Y cells with FITC-labelled p75 antibody clone 8J2 (2 µg/mL). Magnification: 40x.

  • Immunofluorescent staining of internalized NGFR/p75 receptor (green) in rat C6 glioma cells. Live cells were incubated with FITC-labelled NGFR/p75 antibody clone (2 µg/mL) for 1 hour at 37C, fixed (4% formaldehyde), and cell nuclei stained with DAPI (blue). Confocal images (60x magnification) demonstrate typical vesicle-like, punctuate perinuclear staining of internalized receptor/antibody.

    Citations & References

  • General References Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. J. Neurosci. Res. 86(3) pp. 553-65.

    Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR. J. Neurosci. 28(6) pp. 1404-9.

    Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. J. Neurosci. 27(30) pp. 7939-53.