Capsaicin receptor (TrpV1), Rabbit Polyclonal Antibody

Product Info

Product Name Capsaicin receptor (TrpV1), Rabbit Polyclonal Antibody
Product Description google Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Alternative Names Transient receptor potential cation channel subfamily V member 1; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; TRPV1; VR1; VR-1;
Application(s) IHC-Frozen
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity Specificity was confirmed by IHC using frozen sections of rat dorsal root ganglia (DRG) and spinal cord. Due to sequence homology, similar staining is predicted in human DRG and spinal cord. Human, rat. Other species have not yet been tested.
Species Reactivity Human, Rat
Immunogen Description A synthetic peptide (PSESTSHRWRGPA) of human capsaicin receptor protein (aa: 608-621) conjugated to KLH has been used as the antigen.
Conjugate Unconjugated
Purity Description Whole serum
Regulatory Status For research use only.

Specifications

Product Description Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Application(s) IHC-Frozen
Application Details IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Capsaicin receptor (TrpV1)
Specificity Specificity was confirmed by IHC using frozen sections of rat dorsal root ganglia (DRG) and spinal cord. Due to sequence homology, similar staining is predicted in human DRG and spinal cord. Human, rat. Other species have not yet been tested.
Target Host Species Human
Species Reactivity Human, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype Mixed
Conjugate Unconjugated
Immunogen Description A synthetic peptide (PSESTSHRWRGPA) of human capsaicin receptor protein (aa: 608-621) conjugated to KLH has been used as the antigen.
Purity Description Whole serum
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Transient receptor potential cation channel subfamily V member 1; TrpV1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; TRPV1; VR1; VR-1;
Uniprot Number Q8NER1
Uniprot Number/Name Q8NER1 (TRPV1_HUMAN)
Scientific Background Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Detection of capsaicin receptor in small neurons in rat dorsal root ganglion (DRG) using rabbit antibody to human capsaicin receptor (608-621): whole serum, catalogue number R-076-100, at 1:2000 dilution. The rat tissue was prepared by perfusion fixation with 4% formaldehyde containing 10% saturated picric acid in buffered saline. Frozen sections (40 µm) were used and the secondary antibody was conjugated with HRP. Rat spinal cord prepared in the same way also shows a high specific stain with little background.

Citations & References

Specific References Jabin Fagelskiold A et al (2012) Insulin-secreting INS-1E cells express functional TRPV1 channels. Islets. 2012 Jan 1;4(1).
General References Caterina et al. Nature 398: 436(1999).
Tominaga et al. Neuron. 21: 531-543(1998).
Nagy, I. et al. Eur J Pharmacol 500:351-369(2004).